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Bacillus cereus is an opportunistic pathogen and an important cause of diarrheal food-borne illness. The primary mode of transmission is through the gastrointestinal tract, and an emphasis has been placed on the virulence potential of B. cereus. This is because of the association of B. cereus with intestinal mucosal damage and diarrheal illness. The capacity to produce emetic and diarrheal toxins is a key feature that distinguishes B. cereus from other Gram-positive organisms, such as Bacillus anthracis, which are primarily enteropathogens. Bacillus cereus is also known to produce the enterotoxin cereulide, a cyclic dipeptide toxin and virulence factor, which might contribute to its pathogenicity. To study the influence of B. cereus cell wall components on the interaction with Escherichia coli and Listeria innocua, the growth and secreted enterotoxin of each bacterium was examined in the presence of different types of B. cereus cell wall components, in comparison with B. cereus strains exhibiting normal (wild-type) phenotype. In this study, an isogenic B. cereus strain, designated PR14, which contains a transposon insertion within the primary cell wall autolysin-like protein gene, was used as a representative strain of the type that does not produce the enterotoxins. According to our results, the isogenic mutant strain, whose cell surface was devoid of the enterotoxin, also inhibited the growth of E. coli and L. innocua, though to a lesser extent. B. cereus strains producing enterotoxin also reduced the growth of these species, in comparison with the wild-type strain. In summary, it was demonstrated that the severity of the inhibitory effect of B. cereus strains may depend upon the structure and relative number of cell wall components produced. The results presented here should be helpful in understanding the role of B. cereus in the occurrence of diarrheal disease.Meier: Dolphins Bleed the Green and Gold
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