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The aim of the present study was to assess, in an ex vivo model, the effects of glucagon-like peptide-1 (GLP-1) or one of its analogues on the function of the bile canalicular membrane. For this purpose, bile canalicular membrane vesicles (BAMV), isolated from the hepatic bile canaliculi of adrenalectomized and cortisone-replaced adult male rats, were used. BAMV were exposed to radiolabeled taurocholate (TC) and the biliary formation of the polar and non-polar components of TC was determined. At 300 nM, GLP-1 strongly inhibited the TC-stimulated transport of non-polar TC (86% inhibition). In contrast, when the level of GLP-1 was increased, the inhibitory effect was more pronounced. The inhibitory effects of GLP-1 were mimicked by the GLP-1 analogue, liraglutide, and the effects of GLP-1 were reproduced by the GLP-1 receptor agonist, exendin-4. GLP-1 analogues may therefore be of potential therapeutic interest in cholestatic liver disease.Q:
What is the relationship between nfs and home directories?
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